一种小麦抗条锈病相关的if2类翻译起始因子基因

IF2 translation initiation factor gene relevant to wheat yellow rust resistance

Abstract

The invention discloses an IF2 translation initiation factor gene relevant to wheat yellow rust resistance. The overall length of a cDNA sequence of the gene is 1810bp, and a nucleotide sequence of the gene is shown as SEQ.ID.NO.1, wherein 1-165bp is a 5' non-translated region; 166-1386bp codes 406 kinds of amino acid, 1513-1665bp codes 50 kinds of amino acid, and 1666-1810bp is a 3' non-translated region. The gene is a conservative gene of wheat yellow rust resistance; RT-PCR expression analysis indicates that the gene has trace expressions under the normal condition, the induction expressionis increased after duress for 24 hours in a CY32 bacterial system, and the expression reaches the maximum for 72 hours and then is reduced in the following 4-7 days; disease resistant wheat leaves can display a symptom of suffering from puccinia striiformis infection by silencing the expression of the gene in yellow rust resistant wheat by an RNA interference technology.
本发明公开了一种小麦抗条锈病相关的IF2类翻译起始因子基因,该基因的cDNA序列全长为1810bp,其核苷酸序列如SEQ.ID.NO.1所示,其中,1~165bp为5’非翻译区,166~1386bp编码406个氨基酸,1513~1665bp编码50个氨基酸,1666~1810bp为3’非翻译区。该基因是小麦抗条锈病的保守基因,RT-PCR表达分析表明,该基因在正常情况下有微量的表达,在条中32菌系胁迫24h后诱导表达上调,72h时表达量达到最大,随后4-7天表达下降;利用RNA干扰技术沉默该基因在抗条锈病小麦中的表达,会使抗病小麦叶片表现受条锈菌侵染症状。

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