通过使用涂敷的造影剂的生物材料显像

Visualization of biological material by the use of coated contrast agents

Abstract

A method for visualizing biological material, preferably by MRI, comprising the steps of: (i) bringing a population of coated nanoparticles into contact with said biological material, each of which nanoparticles comprises a) a metal oxide of a transition metal, said metal oxide preferably being paramagnetic and preferably comprising a lanthanide (+III) such as gadolinium (+III), and b) a coating covering the surface of the core particle, and (ii) recording the image; wherein the coating is hydrophilic and comprises a silane layer which is located next to the surface of the core particle and comprises one or more different silane groups which each comprises an organic group R and a silane- siloxane linkage where a) R comprises a hydrophilic organic group R' and a hydrophobic spacer B, b) Ois oxygen directly binding to a surface metal ion of the metal oxide, and c) C is carbon and is also part of B. A composition for visualization and methods for the manufacture of the nanoparticles andcore particles are also disclosed. Visualization includes imaging by MR, CT, X-ray, near IR fluorescence, PET, microscopying etc with the largest advantages accomplished for in-vivo imaging.
一种用于使生物材料显像的方法,优选通过MRI,包括以下步骤:(i)使一个群组的经涂敷的纳米颗粒与所述生物材料相接触,这些纳米颗粒各自包括a)一种过渡金属的金属氧化物,所述金属氧化物优选是顺磁性的并且优选地包括一种镧系元素(+III)如钆(+III),以及b)覆盖核心颗粒表面的一个涂层,并且(ii)记录图像;其中该涂层是亲水的并且包括一个硅烷层,该硅烷层位于紧靠该核心颗粒表面处并且包括一个或多个不同的硅烷基团,这些硅烷基团各自包括一个有机的基团R以及一个硅烷-硅氧烷连接,其中a)R包括一个亲水的有机基团R′以及一个疏水的间隔基B,b)O是直接结合至该金属氧化物的一个表面金属离子上的氧,并且c)C是碳并且还是B的一部分。还披露了用于显像的一种组合物以及用于制造这些纳米颗粒以及核心颗粒的方法。显像包括通过MR、CT、X射线、近IR荧光、PET、显微术等进行成像,具有的最大优点是实现了活体内成像。

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    CN-103079642-AMay 01, 2013丹麦科技大学纳米颗粒引导的放射疗法